CTNNA3 Recombinant Rabbit Monoclonal Antibody [JE55-32]
cat.: ET7111-07
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE55-32 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 100 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant fragment within C-terminal Human CTNNA3. |
| Positive control: | Rat heart tissue lysate, mouse heart tissue lysate, 293 cell lysate, mouse heart tissue, rat heart tissue. |
| Subcellular location: | Cytoskeleton. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9UI47 Human | Q65CL1 Mouse Entrez Gene: 361839 Rat |
| Alternative names: | 4930429L08Rik 4933408A16 Alpha catenin like protein Alpha T catenin Alpha T-catenin Cadherin associated protein Cadherin-associated protein Catenin alpha 3 Catenin alpha-3 Catna3 CTNA3_HUMAN CTNNA3 MGC26194 MGC75041 OTTHUMP00000019684 OTTMUSP00000028119 OTTMUSP00000028120 RP11-433J16.1 VR22 |
Images
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Fig1:
Western blot analysis of CTNNA3 on different lysates with Rabbit anti-CTNNA3 antibody (ET7111-07) at 1/1,000 dilution. Lane 1: Rat heart tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: 293 cell lysate (10 µg/Lane) Lysates/proteins at 20 µg/Lane. Predicted band size: 100 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-07) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-CTNNA3 antibody (ET7111-07) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-07) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-CTNNA3 antibody (ET7111-07) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-07) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.