CTNNA3 Recombinant Rabbit Monoclonal Antibody [JE55-32]
cat.: ET7111-07
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE55-32 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 100 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant fragment within C-terminal Human CTNNA3. |
| Positive control: | Rat heart tissue lysate, mouse heart tissue lysate, 293 cell lysate, mouse heart tissue, rat heart tissue. |
| Subcellular location: | Cytoskeleton. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9UI47 Human | Q65CL1 Mouse Entrez Gene: 361839 Rat |
| Alternative names: | 4930429L08Rik 4933408A16 Alpha catenin like protein Alpha T catenin Alpha T-catenin Cadherin associated protein Cadherin-associated protein Catenin alpha 3 Catenin alpha-3 Catna3 CTNA3_HUMAN CTNNA3 MGC26194 MGC75041 OTTHUMP00000019684 OTTMUSP00000028119 OTTMUSP00000028120 RP11-433J16.1 VR22 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-CTNNA3 antibody (ET7111-07) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-07) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-CTNNA3 antibody (ET7111-07) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-07) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Western blot analysis of CTNNA3 on different lysates with Rabbit anti-CTNNA3 antibody (ET7111-07) at 1/1,000 dilution. Lane 1: HEK-293 (Human embryonic kidney cell) cell lysate Lane 2: Mouse testis tissue lysate Lane 3: Rat testis tissue lysate Lysates/proteins at cell:20ug/lane,tissue:40ug/lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7111-07, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 99.8 kDa Observed band size: 100 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.