GTF2F2 Recombinant Rabbit Monoclonal Antibody [JE56-15]
cat.: ET7111-08
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE56-15 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 28 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal Human GTF2F2. |
| Positive control: | Rat testis tissue lysate, A549 cell lysate, HepG2 cell lysate, HL-60 cell lysate, THP-1 cell lysate, Daudi cell lysate, rat testis tissue, human skin tissue, human placenta tissue, human uterus tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P13984 Human | Q01750 Rat |
| Alternative names: | ATP dependent helicase GTF2F2 ATP-dependent helicase GTF2F2 BTF4 General transcription factor IIF 30 kDa subunit General transcription factor IIF polypeptide 2 General transcription factor IIF subunit 2 General transcription factor IIF, polypeptide 2, 30kDa Gtf2f2 RAP30 T2FB_HUMAN TF2F2 TFIIF TFIIF beta TFIIF-beta Transcription initiation factor IIF subunit beta Transcription initiation factor RAP30 |
Images
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Fig1:
Western blot analysis of GTF2F2 on different lysates with Rabbit anti-GTF2F2 antibody (ET7111-08) at 1/1,000 dilution. Lane 1: Rat testis tissue lysate Lane 2: A549 cell lysate Lane 3: HepG2 cell lysate Lane 4: HL-60 cell lysate Lane 5: THP-1 cell lysate Lane 6: Daudi cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-08) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-GTF2F2 antibody (ET7111-08) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-08) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-GTF2F2 antibody (ET7111-08) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-08) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-GTF2F2 antibody (ET7111-08) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-08) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human uterus tissue with Rabbit anti-GTF2F2 antibody (ET7111-08) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-08) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.