Ube2B Recombinant Rabbit Monoclonal Antibody [JE56-12]
cat.: ET7111-09
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Zebrafish
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE56-12
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 17 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal Human Ube2B.
Positive control: HepG2 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, zebrafish tissue lysates, human breast carcinoma tissue, human fetal skeletal muscle tissue.
Subcellular location: Cell membrane, Nucleus.
Recommended Dilutions:
  WB
  IHC-P
1:500-1:5,000
1:50-1:200
Uniprot #: SwissProt: P63146 Human | P63147 Mouse | P63149 Rat
Alternative names: E2 17kDa E2 protein hHR6B HR6B RAD6 homolog B RAD6B UBC2 UBE2B UBE2B_HUMAN Ubiquitin carrier protein B Ubiquitin conjugating enzyme E2 B Ubiquitin conjugating enzyme E2B (RAD6 homolog) Ubiquitin conjugating enzyme E2B Ubiquitin protein ligase B Ubiquitin-conjugating enzyme E2 17 kDa Ubiquitin-conjugating enzyme E2 B Ubiquitin-conjugating enzyme E2-17 kDa Ubiquitin-protein ligase B
Images
ET7111-09_1.jpg Fig1: Western blot analysis of Ube2B on different lysates with Rabbit anti-Ube2B antibody (ET7111-09) at 1/5,000 dilution.

Lane 1: HepG2 cell lysate (10 µg/Lane)
Lane 2: Jurkat cell lysate (10 µg/Lane)
Lane 3: NIH/3T3 cell lysate (10 µg/Lane)
Lane 4: PC-12 cell lysate (10 µg/Lane)
Lane 5: Mouse testis tissue lysate (20 µg/Lane)
Lane 6: Rat testis tissue lysate (20 µg/Lane)

Predicted band size: 17 kDa
Observed band size: 17 kDa

Exposure time: 10 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-09) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7111-09_2.jpg Fig2: Western blot analysis of Ube2B on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-09, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET7111-09_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Ube2B antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-09, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7111-09_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Ube2B antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-09, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.