UBE4B Recombinant Rabbit Monoclonal Antibody [JE55-76]
cat.: ET7111-11
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE55-76 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 146 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal Human UBE4B. |
| Positive control: | HepG2 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, rat skeletal muscle tissue, human lung carcinoma tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: O95155 Human | Q9ES00 Mouse Entrez Gene: 298652 Rat |
| Alternative names: | 4930551I19Rik 4933406G05Rik AU014668 D4Bwg0973e E4 HDNB1 Homozygously deleted in neuroblastoma 1 KIAA0684 mKIAA0684 RP23 173D8.1 Ube4b UBE4B_HUMAN Ubiquitin conjugation factor E4 B Ubiquitin fusion degradation protein 2 UBOX3 UFD2 UFD2a Ufd2p |
Images
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Fig1:
Western blot analysis of UBE4B on different lysates with Rabbit anti-UBE4B antibody (ET7111-11) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 146 kDa Observed band size: 146 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-11) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2:
All lanes: Western blot analysis of UBE4B with anti-UBE4B antibody [JE55-76] (ET7111-11) at 1:1,000 dilution. Lane 1: Wild-type N2A whole cell lysate (30 µg). Lane 2: UBE4B knockout N2A whole cell lysate (30 µg). ET7111-11 was shown to specifically react with UBE4B in wild-type N2A cells. No band was observed when UBE4B knockdout sample was tested. Wild-type and UBE4B knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET7111-11, 1:1,000) was used in 5% BSA at room temperature for 2 hours. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-UBE4B antibody (ET7111-11) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-11) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-UBE4B antibody (ET7111-11) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-11) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.