HTF9C Recombinant Rabbit Monoclonal Antibody [JE55-60]
cat.: ET7111-14
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE55-60 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 69 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant fragment within N-terminal Human HTF9C. |
| Positive control: | 293 cell lysate, Jurkat cell lysate, MCF-7 cell lysate, human liver carcinoma tissue, human thyroid tissue, human breast carcinoma tissue, 293. |
| Subcellular location: | Nucleoplasm and Cytosol.(Predicted) |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:500-1:1,000 1:50-1:200 1:100 |
| Uniprot #: | SwissProt: Q8IZ69 Human |
| Alternative names: | HpaII tiny fragments locus 9c protein HTF9C TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae) TRM2 tRNA methyltransferase 2 homolog A TRM2A_HUMAN TRMT2A tRNA (uracil-5-)-methyltransferase homolog A tRNA methyltransferase 2 homolog A tRNA methyltransferase 2, S. cerevisiae, homolog of, A |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-HTF9C antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-14, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-HTF9C antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-14, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-HTF9C antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-14, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of 293 cells labeling HTF9C with Rabbit anti-HTF9C antibody (ET7111-14) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HTF9C antibody (ET7111-14) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Western blot analysis of HTF9C on different lysates with Rabbit anti-HTF9C antibody (ET7111-14) at 1/2,000 dilution. Lane 1: HEK-293(Human embryonic kidney cell) cell lysate Lane 2: 293T(Human embryonic kidney cell) cell lysate+ Lane 3: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 4: MCF-7 (Human breast cancer cell) cell lysate Lane 5: HepG2 (Human hepatocellular carcinoma cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7111-14, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 69 kDa Observed band size: 75 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.