Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE55-39 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 30 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within N-terminal Human Pirh2. |
Positive control: | HeLa cell lysate, 293T cell lysate, RAW264.7 cell lysate. |
Subcellular location: | Nucleus, Nucleus speckle, Cytoplasm. |
Recommended Dilutions:
WB |
1:500-1:2,000 |
Uniprot #: | SwissProt: Q96PM5 Human | Q9CR50 Mouse |
Alternative names: | Androgen receptor N terminal interacting protein Androgen receptor N-terminal-interacting protein ARNIP CH-rich-interacting match with PLAG1 CHIMP E3 ubiquitin-protein ligase Pirh2 hARNIP hPirh2 p53 induced protein with a RING H2 domain p53-induced RING-H2 protein PIRH2E PIRH2F PRO1996 RCHY1 Ring finger and CHY zinc finger domain containing 1 E3 ubiquitin protein ligase RING finger and CHY zinc finger domain-containing protein 1 RING finger protein 199 RNF199 ZCHY ZFP 363 zinc finger CHY type Zinc finger protein 363 ZN363_HUMAN ZNF363 |
Fig1:
Western blot analysis of Pirh2 on different lysates with Rabbit anti-Pirh2 antibody (ET7111-16) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: RAW264.7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 30 kDa Observed band size: 27 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-16) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |