Pirh2 Recombinant Rabbit Monoclonal Antibody [JE55-39]
cat.: ET7111-16
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB
Clonality: Monoclonal
Clone number: JE55-39
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 30 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal Human Pirh2.
Positive control: HeLa cell lysate, 293T cell lysate, RAW264.7 cell lysate.
Subcellular location: Nucleus, Nucleus speckle, Cytoplasm.
Recommended Dilutions:
  WB

1:500-1:2,000
Uniprot #: SwissProt: Q96PM5 Human | Q9CR50 Mouse
Alternative names: Androgen receptor N terminal interacting protein Androgen receptor N-terminal-interacting protein ARNIP CH-rich-interacting match with PLAG1 CHIMP E3 ubiquitin-protein ligase Pirh2 hARNIP hPirh2 p53 induced protein with a RING H2 domain p53-induced RING-H2 protein PIRH2E PIRH2F PRO1996 RCHY1 Ring finger and CHY zinc finger domain containing 1 E3 ubiquitin protein ligase RING finger and CHY zinc finger domain-containing protein 1 RING finger protein 199 RNF199 ZCHY ZFP 363 zinc finger CHY type Zinc finger protein 363 ZN363_HUMAN ZNF363
Images
ET7111-16_1.jpg Fig1: Western blot analysis of Pirh2 on different lysates with Rabbit anti-Pirh2 antibody (ET7111-16) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: RAW264.7 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 30 kDa
Observed band size: 27 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-16) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.