HLA-DRB4 Recombinant Rabbit Monoclonal Antibody [JE55-85]
cat.: ET7111-17
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JE55-85
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 30 kDa
Isotype: IgG
Immunogen: Synthetic peptide corresponding to the extracellular domain of Human HLA-DRB4.
Positive control: Raji cell lysates, human tonsil tissue, human spleen tissue, human breast carcinoma tissue, Jurkat.
Subcellular location: Lysosome membrane, Endosome membrane, Late endosome membrane, trans-Golgi network membrane, Endoplasmic reticulum membrane, Cell membrane.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500-1:2,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P13762 Human
Alternative names: DR 4 DR beta 4 chain DR4 DRB1 transplantation antigen DRB4 DRB4_HUMAN HLA class II histocompatibility antigen HLA class II histocompatibility antigen DR beta 4 chain HLA-DRB4 Human leucocyte antigen DRB4 Leukocyte antigen Major histocompatibility complex class II DR beta 4 MHC class II antigen DRB4 MHC class II antigen HLA DR beta MHC class2 antigen MHC HLA DR-beta chain
Images
ET7111-17_1.jpg Fig1: Western blot analysis of HLA-DRB4 on Raji cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-17, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET7111-17_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HLA-DRB4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-17, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7111-17_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-HLA-DRB4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-17, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7111-17_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-HLA-DRB4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-17, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7111-17_5.jpg Fig5: Flow cytometric analysis of HLA-DRB4 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7111-17, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.