SerpinB3 Recombinant Rabbit Monoclonal Antibody [JE55-93]
cat.: ET7111-21
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE55-93 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within N-terminal Human SerpinB3. |
| Positive control: | A431 cell lysates, human skin tissue, human esophagus tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:200 |
| Uniprot #: | SwissProt: P29508 Human |
| Alternative names: | HsT1196 Protein T4 A Protein T4-A SCC SCCA SCCA PD SCCA-1 SCCA-PD SCCA1 SCCAPD Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 3 Serpin B3 Serpin peptidase inhibitor clade B (ovalbumin) member 3 serpin peptidase inhibitor, clade B (ovalbumin), member 3 SERPINB3 SPB3_HUMAN Squamous cell carcinoma antigen 1 T4 A T4-A T4A |
Images
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Fig1:
Western blot analysis of SerpinB3 on A431 cell lysates with Rabbit anti-SerpinB3 antibody (ET7111-21) at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-21) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-SerpinB3 antibody (ET7111-21) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-21) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-SerpinB3 antibody (ET7111-21) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-21) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.