CC2D1A Recombinant Rabbit Monoclonal Antibody [JE55-34]
cat.: ET7111-22
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE55-34 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 104 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within C-terminal Human CC2D1A . |
| Positive control: | MCF-7 cell lysate, A431 cell lysate, PC-12 cell lysate, human appendix tissue, human tonsil tissue, MCF-7. |
| Subcellular location: | Centrosome, Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q6P1N0 Human | Q66HA5 Rat |
| Alternative names: | Akt kinase interacting protein 1 Coiled coil and C2 domain containing 1A Coiled coil and C2 domain containing protein 1A Five repressor element under dual repression binding protein 1 FLJ20241 FLJ41160 FRE under dual repression binding protein 1 FREUD 1 Freud 1/Aki1 Mental retardation, nonsyndromic, autosomal recessive, 3 MRT3 Putative NF kappa B activating protein 023N Putative NF kappa B activating protein |
Images
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Fig1:
Western blot analysis of CC2D1A on different lysates with Rabbit anti-CC2D1A antibody (ET7111-22) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-CC2D1A KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 104 kDa Observed band size: 130 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-22) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of CC2D1A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-22, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: A431 cell lysate Lane 3: PC-12 cell lysate |
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Fig3: Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-CC2D1A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-22, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CC2D1A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-22, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of CC2D1A was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7111-22, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.