NAT10 Recombinant Rabbit Monoclonal Antibody [JE55-38]
cat.: ET7111-23
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JE55-38
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 116 kDa
Isotype: IgG
Immunogen: Recombinant protein within C-terminal Human NAT10.
Positive control: HL-60 cell lysate, Daudi cell lysate, K562 cell lysate, human lung carcinoma tissue, human colon carcinoma tissue, mouse brain tissue, Daudi.
Subcellular location: Nucleolus, Midbody.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500-1:2,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q9H0A0 Human | Q8K224 Mouse
Alternative names: ALP DKFZp434C116 FLJ10774 FLJ12179 FLJ23850 hALP KIAA1709 N acetyltransferase 10 N acetyltransferase 10 GCN5 related N acetyltransferase like N acetyltransferase like protein N-acetyltransferase 10 NAT10 NAT10_HUMAN NET43
Images
ET7111-23_1.jpg Fig1: Western blot analysis of NAT10 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-23, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HL-60 cell lysate
Lane 2: Daudi cell lysate
Lane 3: K562 cell lysate
ET7111-23_2.jpg Fig2: Western blot analysis of NAT10 on different lysates with Rabbit anti-NAT10 antibody (ET7111-23) at 1/500 dilution.

Lane 1: Hela-si NT cell lysate
Lane 2: Hela-si NAT10 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 116 kDa
Observed band size: 116 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

ET7111-23 was shown to specifically react with NAT10 in Hela-si NT cells. Weakened band was observed when Hela-si NAT10 sample was tested. Hela-si NT and Hela-si NAT10 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET7111-23, 1/500) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
ET7111-23_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-NAT10 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-23, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7111-23_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-NAT10 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-23, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7111-23_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NAT10 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-23, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7111-23_6.jpg Fig6: Flow cytometric analysis of NAT10 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7111-23, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.