Keratin 12/K12 Recombinant Rabbit Monoclonal Antibody [JE55-35]
cat.: ET7111-24
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE55-35 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 54 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within C-terminal Human Keratin 12/K12. |
| Positive control: | Mouse eyeball tissue lysates, rat eyeball tissue. |
| Subcellular location: | Intermediate filament, cytosol, extracellular exosome. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q99456 Human | Q64291 Mouse | Q6IFW5 Rat |
| Alternative names: | CK-12 Cytokeratin-12 K12 K1C12_HUMAN Keratin 12 Keratin Keratin, type I cytoskeletal 12 Keratin-12 KRT12 type I cytoskeletal 12 |
Images
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Fig1: Western blot analysis of Keratin 12/K12 on mouse eyeball tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-24, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded rat eyeball tissue using anti-Keratin 12/K12 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-24, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.