Desmocollin 3 Recombinant Rabbit Monoclonal Antibody [JE55-86]
cat.: ET7111-26
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: JE55-86
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 100 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal Human Desmocollin 3.
Positive control: Rat skin tissue lysate, A431 cell lysate, 293T, human skin tissue, mouse esophagus tissue, A431.
Subcellular location: Cell membrane, desmosome.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:500-1:2,000
1:50-1:100
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q14574 Human | P55850 Mouse
Alternative names: Cadherin family member 3 CDHF3 desmocollin 3a/3b Desmocollin 4 Desmocollin-3 Desmocollin-4 Desmocollin3 Desmocollin4 DSC DSC1 DSC2 DSC3 DSC3_HUMAN DSC4 HT CP HT-CP HTCP
Images
ET7111-26_1.jpg Fig1: Western blot analysis of Desmocollin 3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-26, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: rat skin tissue lysate
Lane 2: A431 cell lysate
ET7111-26_2.jpg Fig2: ICC staining of Desmocollin 3 in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7111-26, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7111-26_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Desmocollin 3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-26, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7111-26_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse esophagus tissue using anti-Desmocollin 3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-26, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7111-26_5.jpg Fig5: Flow cytometric analysis of Desmocollin 3 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7111-26, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.