Desmocollin 3 Recombinant Rabbit Monoclonal Antibody [JE55-86]
cat.: ET7111-26
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE55-86 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 100 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal Human Desmocollin 3. |
| Positive control: | A431 cell lysate, HCT 116 cell lysate, mouse skin tissue lysate, rat skin tissue lysate, human skin tissue, mouse esophagus tissue. |
| Subcellular location: | Cell membrane, desmosome. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q14574 Human | P55850 Mouse Entrez Gene: 307563 Rat |
| Alternative names: | Cadherin family member 3 CDHF3 desmocollin 3a/3b Desmocollin 4 Desmocollin-3 Desmocollin-4 Desmocollin3 Desmocollin4 DSC DSC1 DSC2 DSC3 DSC3_HUMAN DSC4 HT CP HT-CP HTCP |
Images
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Fig1:
Western blot analysis of Desmocollin 3 on different lysates with Rabbit anti-Desmocollin 3 antibody (ET7111-26) at 1/2,000 dilution. Lane 1: A431 cell lysate (RIPA lysis) Lane 2: HCT 116 cell lysate (low expression) (RIPA lysis) Lane 3: Mouse skin tissue lysate (RIPA lysis) Lane 4: Mouse skin tissue lysate (hot lysis) Lane 5: Rat skin tissue lysate (hot lysis) Lysates/proteins at 20 µg/Lane. Predicted band size: 100 kDa Observed band size: 100 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-26) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Desmocollin 3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-26, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse esophagus tissue using anti-Desmocollin 3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-26, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.