UCHL3 Recombinant Rabbit Monoclonal Antibody [JE55-94]
cat.: ET7111-28
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE55-94 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal Human UCHL3. |
| Positive control: | Daudi cell lysate, HeLa cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, Mouse colon tissue lysate, Rat heart tissue lysate, Rat testis tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P15374 Human | Q9JKB1 Mouse | Q91Y78 Rat |
| Alternative names: | Ubiquitin carboxyl-terminal hydrolase isozyme L3 Ubiquitin thioesterase L3 Ubiquitin thiolesterase Ubiquitin thiolesterase L3 UCH L3 UCH-L3 UCHL3 UCHL3_HUMAN |
Images
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Fig1:
Western blot analysis of UCHL3 on different lysates with Rabbit anti-UCHL3 antibody (ET7111-28) at 1/1,000 dilution. Lane 1: Daudi cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: Neuro-2a cell lysate (20 µg/Lane) Lane 4: PC-12 cell lysate (20 µg/Lane) Lane 5: Mouse colon tissue lysate (40 µg/Lane) Lane 6: Rat heart tissue lysate (40 µg/Lane) Lane 7: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-28) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of UCHL3 on different lysates with Rabbit anti-UCHL3 antibody (ET7111-28) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-UCHL3 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 180 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-28) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.