MRPL28 Recombinant Rabbit Monoclonal Antibody [JE55-90]
cat.: ET7111-30
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Zebrafish
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: JE55-90
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 30 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal Human MRPL28.
Positive control: Mouse liver tissue lysate, SW480 cell lysate, rat stomach tissue lysate, human skin tissue lysate, A549 cell lysate, K562 cell lysate, Hela cell lysate, zebrafish tissue lysates, 293T, MCF-7, SW480, human tonsil tissue, human liver tissue, human liver carcinoma tissue.
Subcellular location: Mitochondrion.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:2,000
1:50-1:100
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q13084 Human | Q9D1B9 Mouse
Alternative names: 39S ribosomal protein L28 39S ribosomal protein L28 mitochondrial 39S ribosomal protein L28 mitochondrial precursor HGNC6756 L28mt MAAT 1 MAAT1 Melanoma antigen p15 Melanoma associated antigen recognised by cytotoxic T lymphocytes Melanoma associated antigen recognized by T lymphocytes Melanoma-associated antigen recognized by T-lymphocytes MGC8499 mitochondrial Mitochondrial ribosomal protein L28 MRP L28 MRP-L28 MRPL 28 mrpl28 P15 RM28_HUMAN
Images
ET7111-30_1.jpg Fig1: Western blot analysis of MRPL28 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-30, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse liver tissue lysate
Lane 2: SW480 cell lysate
Lane 3: Rat stomach tissue lysate
Lane 4: Human skin tissue lysate
Lane 5: A549 cell lysate
Lane 6: K562 cell lysate
Lane 7: Hela cell lysate
ET7111-30_2.jpg Fig2: Western blot analysis of MRPL28 on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-30, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET7111-30_3.jpg Fig3: ICC staining of MRPL28 in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7111-30, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7111-30_4.jpg Fig4: ICC staining of MRPL28 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7111-30, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7111-30_5.jpg Fig5: ICC staining of MRPL28 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7111-30, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7111-30_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MRPL28 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-30, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7111-30_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MRPL28 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-30, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7111-30_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-MRPL28 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-30, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7111-30_9.jpg Fig9: Flow cytometric analysis of MRPL28 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7111-30, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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