MRPL28 Recombinant Rabbit Monoclonal Antibody [JE55-90]
cat.: ET7111-30
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE55-90 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 30 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal Human MRPL28. |
| Positive control: | HEK-293 cell lysate, SW620 cell lysate, NIH/3T3 cell lysate, 4T1 cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, zebrafish tissue lysates. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q13084 Human | Q9D1B9 Mouse Entrez Gene: 497876 Rat |
| Alternative names: | 39S ribosomal protein L28 39S ribosomal protein L28 mitochondrial 39S ribosomal protein L28 mitochondrial precursor HGNC6756 L28mt MAAT 1 MAAT1 Melanoma antigen p15 Melanoma associated antigen recognised by cytotoxic T lymphocytes Melanoma associated antigen recognized by T lymphocytes Melanoma-associated antigen recognized by T-lymphocytes MGC8499 mitochondrial Mitochondrial ribosomal protein L28 MRP L28 MRP-L28 MRPL 28 mrpl28 P15 RM28_HUMAN |
Images
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Fig1:
Western blot analysis of MRPL28 on different lysates with Rabbit anti-MRPL28 antibody (ET7111-30) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: SW620 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: 4T1 cell lysate Lane 5: Mouse kidney tissue lysate Lane 6: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 30 kDa Observed band size: 30 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-30) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Western blot analysis of MRPL28 on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-30, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.