ALKBH1 Recombinant Rabbit Monoclonal Antibody [JE56-09]
cat.: ET7111-31
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Zebrafish |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE56-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 44 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal Human ALKBH1. |
| Positive control: | Human placenta tissue lysate, MCF-7 cell lysate, Daudi cell lysate, A549 cell lysate, zebrafish tissue lysates, human liver carcinoma tissue, human skin tissue, human small intestine tissue. |
| Subcellular location: | Mitochondrion, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q13686 Human |
| Alternative names: | ABH ABH1 alkB alkB homolog 1 histone H2A dioxygenase AlkB, alkylation repair homolog 1 (E. coli) ALKB1_HUMAN ALKBH ALKBH1 Alkylated DNA repair protein alkB homolog 1 Alkylation repair homolog 1 Alkylation repair, alkB homolog Alpha-ketoglutarate-dependent dioxygenase ABH1 DNA lyase ABH1 DNA oxidative demethylase ALKBH1 hABH |
Images
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Fig1:
Western blot analysis of ALKBH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-31, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: human placenta tissue lysate Lane 2: MCF-7 cell lysate Lane 2: Daudi cell lysate Lane 2: A549 cell lysate |
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Fig2: Western blot analysis of ALKBH1 on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-ALKBH1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-31, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-ALKBH1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-31, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-ALKBH1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-31, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.