eIF2A Recombinant Rabbit Monoclonal Antibody [JE58-18]
cat.: ET7111-34
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE58-18 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 65 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human EIF2A aa 435-585. |
| Positive control: | A431 cell lysate, PC-12 cell lysate, Rat pancreas tissue lysate, NIH/3T3 cell lysate, Mouse pancreas tissue lysate, human lung carcinoma tissue. |
| Subcellular location: | Cytoplasm, extracellular region or secreted. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9BY44 Human | Q8BJW6 Mouse | P68101 Rat |
| Alternative names: | "65 kDa eukaryotic translation initiation factor 2A CDA 02 CDA02 EIF 2 EIF 2A eIF-2A EIF2 eif2a EIF2A_HUMAN Eukaryotic translation initiation factor 2A 65kDa Eukaryotic translation initiation factor 2A MST089 MSTP004 MSTP089 " |
Images
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Fig1:
Western blot analysis of eIF2A on different lysates with Rabbit anti-eIF2A antibody (ET7111-34) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: PC-12 cell lysate (20 µg/Lane) Lane 3: Rat pancreas tissue lysate (40 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: Mouse pancreas tissue lysate (40 µg/Lane) Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 24 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-34) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of eIF2A on different lysates with Rabbit anti-eIF2A antibody (ET7111-34) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-eIF2A KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-34) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-eIF2A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-34, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of NIH/3T3 cells labeling eIF2A. Cells were fixed and permeabilized. Then stained with the primary antibody (ET7111-34, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
Flow cytometric analysis of PC-12 cells labeling eIF2A. Cells were fixed and permeabilized. Then stained with the primary antibody (ET7111-34, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.