Anti-EIF2A antibody [JE58-18]
cat.: ET7111-34
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE58-18
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 65 kDa
Isotype: IgG
Immunogen: Recombinant protein within human EIF2A aa 435-585.
Positive control: A431 cell lysates, human lung carcinoma tissue.
Subcellular location: Cytoplasm, extracellular region or secreted.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:1,000
1:50-1:200
Uniprot #: SwissProt: Q9BY44 Human | Q8BJW6 Mouse | P68101 Rat
Alternative names: "65 kDa eukaryotic translation initiation factor 2A CDA 02 CDA02 EIF 2 EIF 2A eIF-2A EIF2 eif2a EIF2A_HUMAN Eukaryotic translation initiation factor 2A 65kDa Eukaryotic translation initiation factor 2A MST089 MSTP004 MSTP089 "
Images
ET7111-34_1.jpg Fig1: Western blot analysis of EIF2A on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-34, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET7111-34_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-EIF2A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-34, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.