PAK3 Recombinant Rabbit Monoclonal Antibody [JE57-39]
cat.: ET7111-35
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE57-39 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PAK3 aa 1-150. |
| Positive control: | SH-SY5Y cell lysate, C6 cell lysate, Mouse brain tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: O75914 Human | Q61036 Mouse | Q62829 Rat |
| Alternative names: | Beta PAK bPAK CDKN1A hPAK3 Mental retardation X linked 30 MRX30 MRX47 Oligophrenin 3 OPHN3 p21 (CDKN1A) activated kinase 3 p21 activated kinase 3 p21 CDKN1A activated kinase 3 P21 protein (Cdc42/Rac) activated kinase 3 PAK-3 PAK3 p21 protein (Cdc42/Rac)-activated kinase 3 PAK3beta Pak65alpha Pak65beta Serine threonine protein kinase PAK 3 Stk4 |
Images
|
Fig1:
Western blot analysis of PAK3 on different lysates with Rabbit anti-PAK3 antibody (ET7111-35) at 1/5,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: C6 cell lysate Lane 3: Mouse brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 51 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-35) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.