PAK3 Recombinant Rabbit Monoclonal Antibody [JE57-39]
cat.: ET7111-35
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE57-39 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 62 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PAK3 aa 1-150. |
| Positive control: | Rat brain tissue lysates, F9. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: O75914 Human | Q61036 Mouse | Q62829 Rat |
| Alternative names: | Beta PAK bPAK CDKN1A hPAK3 Mental retardation X linked 30 MRX30 MRX47 Oligophrenin 3 OPHN3 p21 (CDKN1A) activated kinase 3 p21 activated kinase 3 p21 CDKN1A activated kinase 3 P21 protein (Cdc42/Rac) activated kinase 3 PAK-3 PAK3 p21 protein (Cdc42/Rac)-activated kinase 3 PAK3beta Pak65alpha Pak65beta Serine threonine protein kinase PAK 3 Stk4 |
Images
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Fig1: Western blot analysis of PAK3 on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-35, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2: ICC staining of PAK3 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7111-35, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.