PAK3 Recombinant Rabbit Monoclonal Antibody [JE57-39]
cat.: ET7111-35
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Mouse, Rat, Human
Applications: WB, IF-Cell
Clonality: Monoclonal
Clone number: JE57-39
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 62 kDa
Isotype: IgG
Immunogen: Recombinant protein within human PAK3 aa 1-150.
Positive control: Rat brain tissue lysates, F9.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell

1:500-1:1,000
1:50-1:100
Uniprot #: SwissProt: O75914 Human | Q61036 Mouse | Q62829 Rat
Alternative names: Beta PAK bPAK CDKN1A hPAK3 Mental retardation X linked 30 MRX30 MRX47 Oligophrenin 3 OPHN3 p21 (CDKN1A) activated kinase 3 p21 activated kinase 3 p21 CDKN1A activated kinase 3 P21 protein (Cdc42/Rac) activated kinase 3 PAK-3 PAK3 p21 protein (Cdc42/Rac)-activated kinase 3 PAK3beta Pak65alpha Pak65beta Serine threonine protein kinase PAK 3 Stk4
Images
ET7111-35_1.jpg Fig1: Western blot analysis of PAK3 on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-35, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET7111-35_2.jpg Fig2: ICC staining of PAK3 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7111-35, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.