WDR4 Recombinant Rabbit Monoclonal Antibody [JE58-28]
cat.: ET7111-37
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE58-28 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 45 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human WDR4 aa 262-412. |
| Positive control: | K562 cell lysates, human liver carcinoma tissue, human cervical carcinoma tissue, human ovarian tumor tissue, Daudi. |
| Subcellular location: | Nucleus, Chromosome. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P57081 Human |
| Alternative names: | "OTTHUMP00000109401 OTTHUMP00000109402 TRM82 tRNA (guanine N(7) ) methyltransferase subunit WDR4 tRNA (guanine-N(7)-)-methyltransferase subunit WDR4 WD repeat containing protein 4 WD repeat protein 4 WD repeat protein domain 4 protein isoform 2 WD repeat-containing protein 4 wdr4 WDR4_HUMAN " |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-WDR4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-37, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue using anti-WDR4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-37, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human ovarian tumor tissue using anti-WDR4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-37, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of WDR4 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7111-37, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
Western blot analysis of WDR4 on different lysates with Rabbit anti-WDR4 antibody (ET7111-37) at 1/1,000 dilution. Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 60 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7111-37, 1/5,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 45 kDa Observed band size: 45 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.