TrkB Recombinant Rabbit Monoclonal Antibody [JE58-22]
cat.: ET7111-38
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE58-22 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 92 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TrkB aa 100-450/822. |
| Positive control: | Mouse brain tissue lysate, Rat brain tissue lysate, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Cell membrane, Endosome membrane, Early endosome membrane, axon, dendrite, perinuclear region, postsynaptic density. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:200 |
| Uniprot #: | SwissProt: P15209 Mouse | Q63604 Rat |
| Alternative names: | AI848316 BDNF tropomyosine receptor kinase B BDNF/NT 3 growth factors receptor BDNF/NT-3 growth factors receptor Brain derived neurotrophic factor receptor C030027L06Rik EC 2.7.10.1 GP145 TrkB GP145-TrkB GP145-TrkB/GP95-TrkB GP95 TrkB Neurotrophic receptor tyrosine kinase 2 Neurotrophic tyrosine kinase receptor type 2 Neurotrophin receptor tyrosine kinase type 2 NTRK 2 Ntrk2 NTRK2_HUMAN Obesity, hyperphagia, and developmental delay, included RATTRKB1 Tkrb Trk B Trk-B TRKB TrkB tyrosine kinase TRKB1 Tropomyosin related kinase B tyrosine kinase receptor B Tyrosine receptor kinase B |
Images
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Fig1:
Western blot analysis of TrkB on different lysates with Rabbit anti-TrkB antibody (ET7111-38) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse liver tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 92 kDa Observed band size: 92 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-38) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-TrkB antibody (ET7111-38) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-38) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Rabbit anti-TrkB antibody (ET7111-38) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-38) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-TrkB antibody (ET7111-38) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-38) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.