TrkB Recombinant Rabbit Monoclonal Antibody [JE58-22]
cat.: ET7111-38
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-P, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | JE58-22 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 92 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TrkB aa 100-450/822. |
| Positive control: | Mouse brain tissue lysate, Rat brain tissue lysate, Mouse hippocampus tissue lysate, Mouse cortex tissue lysate, Mouse cerebellum tissue lysate, Rat hippocampus tissue lysate, Rat cerebellum tissue lysate, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Cell membrane, Endosome membrane, Early endosome membrane, axon, dendrite, perinuclear region, postsynaptic density. |
| Recommended Dilutions:
WB IHC-P IHC-Fr |
1:2,000-1:5,000 1:200 1:200 |
| Uniprot #: | SwissProt: P15209 Mouse | Q63604 Rat |
| Alternative names: | AI848316 BDNF tropomyosine receptor kinase B BDNF/NT 3 growth factors receptor BDNF/NT-3 growth factors receptor Brain derived neurotrophic factor receptor C030027L06Rik EC 2.7.10.1 GP145 TrkB GP145-TrkB GP145-TrkB/GP95-TrkB GP95 TrkB Neurotrophic receptor tyrosine kinase 2 Neurotrophic tyrosine kinase receptor type 2 Neurotrophin receptor tyrosine kinase type 2 NTRK 2 Ntrk2 NTRK2_HUMAN Obesity, hyperphagia, and developmental delay, included RATTRKB1 Tkrb Trk B Trk-B TRKB TrkB tyrosine kinase TRKB1 Tropomyosin related kinase B tyrosine kinase receptor B Tyrosine receptor kinase B |
Images
|
Fig1:
Western blot analysis of TrkB on different lysates with Rabbit anti-TrkB antibody (ET7111-38) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate (20 µg/Lane) Lane 2: Rat brain tissue lysate (20 µg/Lane) Lane 3: Mouse liver tissue lysate (negative) (20 µg/Lane) Predicted band size: 92 kDa Observed band size: 140/90 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-38) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of TrkB on different lysates with Rabbit anti-TrkB antibody (ET7111-38) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate (40 µg/Lane) Lane 2: Mouse hippocampus tissue lysate (40 µg/Lane) Lane 3: Mouse cortex tissue lysate (40 µg/Lane) Lane 4: Mouse cerebellum tissue lysate (40 µg/Lane) Lane 5: Rat brain tissue lysate (40 µg/Lane) Lane 6: Rat hippocampus tissue lysate (40 µg/Lane) Lane 7: Rat cerebellum tissue lysate (40 µg/Lane) Predicted band size: 92 kDa Observed band size: 140/90 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-38) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-TrkB antibody (ET7111-38) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-38) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Rabbit anti-TrkB antibody (ET7111-38) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-38) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-TrkB antibody (ET7111-38) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-38) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: Brain Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: ET7111-38, 1/200, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.