Oct-2 Recombinant Rabbit Monoclonal Antibody [JE58-05]
cat.: ET7111-39
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE58-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant full length protein of human Oct-2. |
| Positive control: | Daudi cell lysates, human tonsil tissue, Daudi. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P09086 Human | Q00196 Mouse |
| Alternative names: | class 2 Lymphoid restricted immunoglobulin octamer binding protein Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 NF A2 Oct 2 Oct-2 Octamer binding transcription factor 2 Octamer-binding protein 2 Octamer-binding transcription factor 2 OTF-2 OTF2 PO2F2_HUMAN POU domain POU domain class 2 transcription factor 2 POU2F2 transcription factor 2 |
Images
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Fig1: Western blot analysis of Oct-2 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-39, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Oct-2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-39, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of Oct-2 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7111-39, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.