EGR1 Recombinant Rabbit Monoclonal Antibody [JE57-62]
cat.: ET7111-41
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE57-62 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human EGR1 aa 1-200. |
| Positive control: | THP-1 treated with 100ng/mL LPS for 3 hours cell lysate, human brain tissue, human thyroid carcinoma tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: P18146 Human |
| Alternative names: | AT225 Early growth response 1 Early growth response protein 1 EGR 1 EGR-1 EGR1 EGR1_HUMAN G0S30 KROX 24 KROX24 Nerve growth factor-induced clone A Nerve growth factor-induced protein A NGFI-A NGFIA TIS8 Transcription factor ETR103 Transcription factor Zif268 ZIF 268 ZIF268 Zinc finger protein 225 Zinc finger protein Krox-24 ZNF225 |
Images
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Fig1:
Western blot analysis of EGR1 on different lysates with Rabbit anti-EGR1 antibody (ET7111-41) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 100ng/mL LPS for 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 58 kDa Observed band size: 75 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-41) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-EGR1 antibody (ET7111-41) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-41) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-EGR1 antibody (ET7111-41) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-41) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.