HDGF Recombinant Rabbit Monoclonal Antibody [JE56-32]
cat.: ET7111-42
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: JE56-32
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 27 kDa.
Isotype: IgG
Immunogen: Recombinant protein within human HDGF aa 50-200.
Positive control: Rat cerebellum tissue lysate, SK-Br-3 cell lysate, A549 cell lysate, mouse cerebellum tissue lysate, F9, human liver carcinoma tissue, human cervix tissue, human colon carcinoma tissue, human breast carcinoma tissue, human esophagus tissue, human stomach carcinoma tissue, human colon tissue, THP-1.
Subcellular location: Nucleus, Cytoplasm, Extracellular exosome.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:1,000
1:50-1:100
1:200-1:1,000
1:50-1:100
Uniprot #: SwissProt: P51858 Human | P51859 Mouse | Q8VHK7 Rat
Alternative names: FLJ96580 HDGF HDGF_HUMAN Hepatoma derived growth factor Hepatoma-derived growth factor High mobility group protein 1 like 2 High mobility group protein 1-like 2 HMG-1L2 HMG1L2
Images
ET7111-42_1.jpg Fig1: Western blot analysis of HDGF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-42, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Rat cerebellum tissue lysate
Lane 2: SK-Br-3 cell lysate
Lane 3: A549 cell lysate
Lane 4: Mouse cerebellum tissue lysate
ET7111-42_2.jpg Fig2: ICC staining of HDGF in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7111-42, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7111-42_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-HDGF antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-42, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7111-42_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human cervix tissue using anti-HDGF antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-42, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7111-42_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-HDGF antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-42, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7111-42_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-HDGF antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-42, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7111-42_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-HDGF antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-42, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7111-42_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-HDGF antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-42, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7111-42_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-HDGF antibody (ET7111-42) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-42) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7111-42_10.jpg Fig10: Flow cytometric analysis of HDGF was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7111-42, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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