FACL4 Recombinant Rabbit Monoclonal Antibody [JE56-33]
cat.: ET7111-43
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: JE56-33
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 79 kDa
Isotype: IgG
Immunogen: Recombinant protein within human FACL4 aa 561-711 (Cytoplasmic).
Positive control: NIH/3T3 cell lysate, HepG2 cell lysate, HUVEC, rat brain tissue, mouse brain tissue, HepG2, Mouse lung tissue lysate.
Subcellular location: Peroxisome membrane, Mitochondrion outer membrane, Microsome membrane, Endoplasmic reticulum membrane, Cell membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:1,000
1:50-1:100
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: O60488 Human | Q9QUJ7 Mouse | O35547 Rat
Alternative names: ACS 4 ACS4 ACSL 4 Acsl4 ACSL4_HUMAN acyl CoA synthetase 4 Acyl CoA synthetase long chain family member 4 FACL 4 FACL4 Fatty acid Coenzyme A ligase fatty acid Coenzyme A ligase long-chain 4 LACS 4 LACS4 Lignoceroyl CoA synthase Long chain 4 long chain acyl CoA synthetase 4 long chain fatty acid CoA ligase 4 long chain fatty acid Coenzyme A ligase 4 Long-chain acyl-CoA synthetase 4 Long-chain-fatty-acid--CoA ligase 4 MRX63 MRX68
Images
ET7111-43_1.jpg Fig1: Western blot analysis of FACL4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-43, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: NIH/3T3 cell lysate
Lane 2: HepG2 cell lysate
ET7111-43_2.jpg Fig2: ICC staining of FACL4 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7111-43, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7111-43_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-FACL4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-43, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7111-43_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-FACL4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-43, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7111-43_5.jpg Fig5: Flow cytometric analysis of FACL4 was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7111-43, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ET7111-43_6.jpg Fig6: Western blot analysis of FACL4 on different lysates with Rabbit anti-FACL4 antibody (ET7111-43) at 1/500 dilution.

Lane 1: NIH/3T3 cell lysate (10 µg/Lane)
Lane 2: Mouse lung tissue lysate (20 µg/Lane)

Predicted band size: 79 kDa
Observed band size: 75 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-43) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.