ACSL4/FACL4 Recombinant Rabbit Monoclonal Antibody [JE56-33]
cat.: ET7111-43
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE56-33 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 79 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human FACL4 aa 561-711 (Cytoplasmic). |
| Positive control: | PC-12 cell lysate, C6 cell lysate, L6 cell lysate, Rat brain tissue lysate, Rat lung tissue lysate, Rat thymus tissue lysate, A549 cell lysate, Mouse brain tissue lysate, mouse brain tissue, mouse lung tissue, rat brain tissue. |
| Subcellular location: | Peroxisome membrane, Mitochondrion outer membrane, Microsome membrane, Endoplasmic reticulum membrane, Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:5,000 1:1,000 |
| Uniprot #: | SwissProt: O60488 Human | Q9QUJ7 Mouse | O35547 Rat |
| Alternative names: | ACS 4 ACS4 ACSL 4 Acsl4 ACSL4_HUMAN acyl CoA synthetase 4 Acyl CoA synthetase long chain family member 4 FACL 4 FACL4 Fatty acid Coenzyme A ligase fatty acid Coenzyme A ligase long-chain 4 LACS 4 LACS4 Lignoceroyl CoA synthase Long chain 4 long chain acyl CoA synthetase 4 long chain fatty acid CoA ligase 4 long chain fatty acid Coenzyme A ligase 4 Long-chain acyl-CoA synthetase 4 Long-chain-fatty-acid--CoA ligase 4 MRX63 MRX68 |
Images
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Fig1:
Western blot analysis of ACSL4/FACL4 on different lysates with Rabbit anti-ACSL4/FACL4 antibody (ET7111-43) at 1/5,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si ACSL4/FACL4 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 79 kDa Observed band size: 75 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-43) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of ACSL4/FACL4 on different lysates with Rabbit anti-ACSL4/FACL4 antibody (ET7111-43) at 1/1,000 dilution. Lane 1: PC-12 cell lysate Lane 2: C6 cell lysate Lane 3: L6 cell lysate Lane 4: Rat brain tissue lysate Lane 5: Rat lung tissue lysate Lane 6: Rat thymus tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 79 kDa Observed band size: 75 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-43) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Western blot analysis of ACSL4/FACL4 on different lysates with Rabbit anti-ACSL4/FACL4 antibody (ET7111-43) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: Mouse brain tissue lysate (40 µg/Lane) Predicted band size: 79 kDa Observed band size: 75 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-43) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ACSL4/FACL4 antibody (ET7111-43) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-43) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-ACSL4/FACL4 antibody (ET7111-43) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-43) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ACSL4/FACL4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-43, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.