HRP Conjugated Goat anti-Rabbit IgG polyclonal Antibody
cat.: HA1001
Product Type: Goat polyclonal IgG, secondary antibodies
Species reactivity: Rabbit
Applications: WB, ELISA, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Isotype: IgG
Immunogen: Rabbit IgG (H+L).
Recommended Dilutions:
  WB
  ELISA
  IHC-P
1:50,000~1:100,000
1:5,000~1:10,000
1:200-1:1,000
Images
HA1001_1.jpg Fig1: Western blot analysis of rabbit anti-GAPDH antibody (ET1601-4,1/2000) and rabbit anti-GST3 antibody (ER1802-63, 1/1000) with K562 Cell and A549 Cell lysates (All lanes proteins at 10 µg per lane). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat anti-rabbit IgG -HRP) (HA1001) was used for 1 hour at room temperature at 1:40,000 (Lane A), 1:80,000 (Lane B), 1:160,000 (Lane C) and 1:320,000 (Lane D).
Exposure time: 60 seconds
HA1001_2.jpg Fig2: Direct ELISA analysis of Goat Anti-Rabbit IgG (H+L) was performed by coating wells of a 96-well plate with 50 µl per well of Rabbit IgG / Human IgG / Mouse IgG / Rat IgG / Guinea pig IgG diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, and incubated with 100 µl per well of Goat Anti-Rabbit IgG (H+L) polyclonal antibody (HA1001) diluted at a concentration of 62.5 ng/mL for 1 hours at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.