HRP Conjugated Goat anti-Rabbit IgG polyclonal Antibody
cat.: HA1001
| Product Type: | Goat polyclonal IgG, secondary antibodies |
|---|---|
| Species reactivity: | Rabbit |
| Applications: | WB, ELISA, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.5%BSA, 50% Glycerol. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Rabbit IgG (H+L). |
| Recommended Dilutions:
WB ELISA IHC-P |
1:50,000~1:100,000 1:5,000~1:10,000 1:100-1:200 |
Images
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Fig1:
Western blot analysis of rabbit anti-GAPDH antibody (ET1601-4,1/2000) and rabbit anti-GST3 antibody (ER1802-63, 1/1000) with K562 Cell and A549 Cell lysates (All lanes proteins at 10 µg per lane). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat anti-rabbit IgG -HRP) (HA1001) was used for 1 hour at room temperature at 1:40,000 (Lane A), 1:80,000 (Lane B), 1:160,000 (Lane C) and 1:320,000 (Lane D). Exposure time: 60 seconds |
|
Fig2:
The Cross-reactions of the secondary antibody (HA1001) with Mouse IgG and Human IgG. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Goat anti-rabbit IgG -HRP (HA1001) was used for 1 hour at room temperature at 1:40,000 (Lane A) and 1:180,000 (Lane B). Exposure time: 2 minutes |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.