HRP Conjugated Rabbit anti-Guinea pig IgG polyclonal Antibody
cat.: HA1021
| Product Type: | Rabbit polyclonal IgG, secondary antibodies |
|---|---|
| Species reactivity: | Guinea pig |
| Applications: | WB, ELISA |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 55/25 kDa |
| Isotype: | IgG |
| Immunogen: | Purified native Guinea Pig IgG. |
| Positive control: | Guinea pig IgG. |
| Recommended Dilutions:
WB ELISA |
1:5,000-1:40,000 1:5,000-1:20,000 |
Images
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Fig1:
Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Rabbit anti-Guinea pig IgG-HRP (HA1021) was used for 1 hour at room temperature at 1:10,000 (Lane 1) and 1:5,000 (Lane 2). Lane 1: Guinea pig (30 ng per lane). Lane 2: Mouse IgG (30 ng per lane). Exposure time: 1 minutes |
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Fig2: Immobilized guinea pig IgG protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA1021) for 1 hour at 25℃. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.