HRP Conjugated Goat anti-Rat IgG(H+L) polyclonal Antibody
cat.: HA1023
Product Type: Goat polyclonal IgG, secondary antibodies
Species reactivity: Rat
Applications: WB, ELISA
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.5%BSA, 40% Glycerol.
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Isotype: IgG
Immunogen: Rat IgG(H+L).
Recommended Dilutions:
  WB
  ELISA

1:5,000
1:5,000- 1:10,000
Images
HA1023_1.jpg Fig1: Western blot analysis of Goat anti-Rat IgG(H+L) on rat IgG with Goat anti-Rat IgG(H+L) antibody (HA1023) at different dilutions.

Lane 1: Rat IgG at 1:1,000 dilution
Lane 2: Rat IgG at 1:5,000 dilution

Lysates/proteins at 50 ng/Lane.

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA1023) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.