| Product Type: | Goat polyclonal IgG, secondary antibodies |
|---|---|
| Species reactivity: | Rat |
| Applications: | WB, ELISA |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.5%BSA, 40% Glycerol. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Rat IgG(H+L). |
| Recommended Dilutions:
WB ELISA |
1:5,000 1:5,000- 1:10,000 |
|
Fig1:
Western blot analysis of Goat anti-Rat IgG(H+L) on rat IgG with Goat anti-Rat IgG(H+L) antibody (HA1023) at different dilutions. Lane 1: Rat IgG at 1:1,000 dilution Lane 2: Rat IgG at 1:5,000 dilution Lysates/proteins at 50 ng/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA1023) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours. |