HRP Conjugated Alpaca anti-Rabbit IgG Fc, Recombinant VHH monoclonal Antibody
cat.: HA1031
| Product Type: | Alpaca monoclonal IgG, secondary antibodies |
|---|---|
| Species reactivity: | Rabbit |
| Applications: | IP, ELISA, IHC-P, WB |
| Clonality: | Monoclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.5%BSA, 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Fc region of Rabbit IgG. |
| Recommended Dilutions:
IP ELISA IHC-P WB |
Use at an assay dependent concentration. 1:5,000-1:20,000 1:100-1:500 1:50,000-1:100,000 |
Images
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Fig1:
Western blot analysis of rabbit anti-GAPDH antibody (ET1601-4,1/2000) and rabbit anti-GST3 antibody (ER1802-63, 1/1000) with K562 Cell and A549 Cell lysates (All lanes proteins at 10 µg per lane). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Alpaca anti-rabbit IgG Fc, recombinant VHH (HRP) (HA1031) was used for 1 hour at room temperature at 1:40,000 (Lane A, E), 1:80,000 (Lane B, F), 1:160,000 (Lane C, G) and 1:320,000 (Lane D, H). Exposure time: 30 seconds |
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Fig2:
The Cross-reactions of the secondary antibody (HA1031) with Rabbit IgG, Mouse IgG and Human IgG. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Alpaca anti-Rabbit IgG FC, Recombinant VHH (HRP) (HA1031) was used for 1 hour at room temperature at 1/50,000. Lane 1: Rabbit IgG (50 ng/Lane) Lane 2: Mouse IgG (50 ng/Lane) Lane 3: Human IgG (50 ng/Lane) Exposure time: 10 seconds. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human Colorectal cancer tissue using rabbit anti-Cytokeratin 18 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-8, 1/200 dilution) for 30 minutes at room temperature. Alpaca anti-rabbit IgG Fc, recombinant VHH (HRP) (HA1031, 1/200 dilution) was used for 30 min at room temperature. Tissues were counterstained with hematoxylin and mounted with DPX. The inset negative control image is secondary-only at 1/200 dilution. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human Kidney tissue using rabbit anti-CD13 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-45, 1/200) for 30 minutes at room temperature. Alpaca anti-rabbit IgG Fc, recombinant VHH (HRP) (HA1031, 1/200 dilution) was used for 30 min at room temperature. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.