| Product Type: | Rabbit polyclonal IgG, secondary antibodies |
|---|---|
| Species reactivity: | Guinea pig |
| Applications: | WB, ELISA |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 55/25 kDa |
| Isotype: | IgG |
| Immunogen: | Purified native Guinea Pig IgG. |
| Positive control: | Guinea pig IgG. |
| Recommended Dilutions:
WB ELISA |
1:1,000-1:5,000 1:1,000-1:2,000 |
|
Fig1:
Western blot analysis of Rabbit anti-Guinea pig IgG on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA1033, 1/5000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Guinea pig (30 ng per lane) Lane 2: Mouse IgG (30 ng per lane) |