| Product Type: | Goat polyclonal IgG, secondary antibodies |
|---|---|
| Species reactivity: | Rat |
| Applications: | WB, ELISA |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Rat IgG(H+L). |
|
Fig1:
Western blot analysis of Rat IgG on different lysates with Goat anti-Rat IgG(H+L) antibody (HA1034) at 1/1,000 dilution. Lane 1: Mouse IgG Lane 2: Rabbit IgG Lane 3: Rat IgG Lane 4: Human IgG Lysates/proteins at 50 ng/Lane. Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA1034) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Rabbit Anti-Goat IgG - HRP Secondary Antibody (HA1005) at 1/50,000 dilution was used for 1 hour at room temperature. |