Goat anti-Rat IgG(H+L) Goat Polyclonal Antibody
cat.: HA1034
Product Type: Goat polyclonal IgG, secondary antibodies
Species reactivity: Rat
Applications: WB, ELISA
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4).
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Isotype: IgG
Immunogen: Rat IgG(H+L).
Images
HA1034_1.jpg Fig1: Western blot analysis of Rat IgG on different lysates with Goat anti-Rat IgG(H+L) antibody (HA1034) at 1/1,000 dilution.

Lane 1: Mouse IgG
Lane 2: Rabbit IgG
Lane 3: Rat IgG
Lane 4: Human IgG

Lysates/proteins at 50 ng/Lane.

Exposure time: 6 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA1034) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Rabbit Anti-Goat IgG - HRP Secondary Antibody (HA1005) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.