Biotin Conjugated Goat anti-rabbit IgG polyclonal Antibody
cat.: HA1101
| Product Type: | Goat polyclonal IgG, secondary antibodies |
|---|---|
| Species reactivity: | Rabbit |
| Applications: | WB, ELISA, IHC-P, IF-Tissue, FC, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Rabbit IgG (H+L). |
| Recommended Dilutions:
WB ELISA IHC-P IF-Tissue FC IF-Cell |
1:5,000-1:20,000 1:20,000-1:400,000 1:500-1:5,000 1:200-1:1,000 1:200-1:1,000 1:200 |
Images
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Fig1:
Western blot analysis of Goat Anti-Rabbit IgG (H+L) on rabbit/mouse/human IgG with Goat anti-rabbit IgG-biotin (HA1101) at different dilutions. Lane 1: Rabbit IgG, 1:5,000 Lane 2: Rabbit IgG, 1:20,000 Lane 3: Mouse IgG, 1:5,000 Lane 4: Mouse IgG, 1:20,000 Lane 5: Human IgG, 1:5,000 Lane 6: Human IgG, 1:20,000 Lysates/proteins at 50 ng/Lane. Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Goat anti-rabbit IgG-biotin (HA1101) at different dilutions were used in 5% NFDM/TBST at 4℃ overnight. Streptavidin-HRP Secondary Antibody at 1/20,000 dilution was used for 1 hour at room temperature. |
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Fig2: HA1101 was tested by direct ELISA, where wells were coated with serially diluted rabbit/mouse/human IgG (1,000-31.25 ng/mL, 4℃, overnight), blocked with 1% BSA (37℃, 1 hour). HA1101 (1/10,000 dilution; 37℃, 45 minutes) was added and detected by Streptavidin-HRP (1/2,000 dilution; 37℃, 30 minutes). Signal was developed by TMB substrate. Data from duplicates; +/- SD. |
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Fig3:
Immunocytochemistry analysis of HeLa cells labeling SP1 with Rabbit anti-SP1 antibody (ET1702-02) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SP1 antibody (ET1702-02) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG (biotin-conjugated, HA1101) was used at 1/200 dilution. Streptavidin-FITC was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.