iFluor™ 594 Conjugated Goat anti-rabbit IgG Goat Polyclonal Antibody
cat.: HA1122
| Product Type: | Goat polyclonal IgG, secondary antibodies |
|---|---|
| Species reactivity: | Rabbit |
| Applications: | IF-Cell, IF-Tissue, FC, IHC-Fr |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Rabbit IgG (H+L). |
| Recommended Dilutions:
IF-Cell IF-Tissue FC IHC-Fr |
1:500-1:1,000 1:500-1:1,000 1:500-1:1,000 1:500-1:1,000 |
Images
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Fig1:
Immunocytochemistry analysis of SKOV-3 cells labeling Vimentin (ET1610-39). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.05% Triton X-100 in PBS for 10 minutes, and then blocked with 2% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody Vimentin (ET1610-39, red) at 1/200 dilution for overnight at 4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/800 dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. |
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Fig2:
Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling Synaptophysin (ET1606-56) and beta Ⅲ tubulin (M0805-8). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Synaptophysin (ET1606-56, red) at 1/200 dilution and beta Ⅲ tubulin (M0805-8, green) at 1/200 dilution at +4℃ overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) and Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) were used as the secondary antibodies at 1/1,000 dilution. DAPI was used as nuclear counterstain. |
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Fig3: Flow cytometric analysis of CaV2.2 was done on SH-SY5Y cells. The cells was washed twice with cold PBS and resuspend. Then incubation with the primary antibody (CaV2.2, 1ug/ml) for 1 hour at +4℃. The secondary antibody Goat Anti-Rabbit IgG H&L iFluor™ 594 (HA1122, red) was used at 1/500 dilution for 30 min at +4℃(dark incubation). Unlabelled sample was used as a control (cells without incubation with primary antibody; green). Other brands of secondary antibodies on the market are used as controls at the same dilution (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.