iFluor™ 647 Conjugated Goat anti-rabbit IgG polyclonal Antibody
cat.: HA1123
| Product Type: | Goat polyclonal IgG, secondary antibodies |
|---|---|
| Species reactivity: | Rabbit |
| Applications: | IF-Cell, IF-Tissue, IHC-Fr, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Rabbit IgG (H+L). |
| Recommended Dilutions:
IF-Cell IF-Tissue IHC-Fr FC |
1:500-1:1,000 1:500-1:1,000 1:500-1:1,000 1:1,000 |
Images
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Fig1:
Immunocytochemistry analysis of Hela cells labeling Vimentin with Rabbit anti-Vimentin antibody (ET1610-39) at 1/400 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 3% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (ET1610-39) at 1/400 dilution in 3% BSA overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 647, HA1123) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
|
Fig2:
Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling Synaptophysin (ET1606-56) and beta Ⅲ tubulin (M0805-8). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Synaptophysin (ET1606-56, red) at 1/200 dilution and beta Ⅲ tubulin (M0805-8, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. iFluor™ 647 conjugate-Goat anti-Rabbit IgG (HA1123) and iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) were used as the secondary antibodies at 1/500 dilution. DAPI was used as nuclear counterstain. |
|
Fig3:
Flow cytometric analysis of HeLa cells labeling beta Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1602-4, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 647 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1123) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.