iFluor™ 488 Conjugated Goat anti-mouse IgG polyclonal Antibody
cat.: HA1125
| Product Type: | Goat polyclonal IgG, secondary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | IF-Cell, IF-Tissue, FC, IHC-Fr |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Mouse IgG (H+L). |
| Recommended Dilutions:
IF-Cell IF-Tissue FC IHC-Fr |
1:500-1:1,000 1:500-1:1,000 1:500-1:1,000 1:500-1:1,000 |
Images
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Fig1:
Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: Retina Sample: Frozen section Antigen retrieval: Not required. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Iba1 (RT1316), 1/1,000, overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1/500, 15 minutes at room temperature. |
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Fig2:
Application: Immunofluorescence (IF-tissue) Species: Mouse Tissue: Cerebral cortex Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Antibody A: 1/200 (Beta III Tubulin, M0805-8, mouse, Green), Antibody B: 1/200 (Synaptophysin, ET1606-56, rabbit, Red), overnight at 4℃. Secondary antibody: Goat anti-Rabbit IgG (iFluor™ 488), HA1121, 1/1,000; Goat anti-Mouse IgG (iFluor™ 594), HA1126, 1/1,000, 1.5 hours at room temperature. |
|
Fig3:
Immunocytochemistry analysis of HepG2 cells labeling eIF-6 with Mouse anti-eIF-6 antibody (HA600051) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-eIF-6 antibody (HA600051) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Application: Flow Cytometry (Intra) Species: Human Sample: Hep G2 (Human hepatocellular carcinoma cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: eIF-6 (HA600051, 1 μg/mL, Red) compared with Mouse IgG Isotype Control (HA601336, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1/1,000, 15 minutes at room temperature. |
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Fig5:
Application: Flow Cytometry Species: Human Sample: Human peripheral blood lymphocytes Primary antibody: CD3 (HA601337, 1 μg/mL, Red) compared with Control, 15 minutes at room temperature. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1/1,000, 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.