iFluor™ 488 Conjugated Alpaca anti-Rabbit IgG FC, Recombinant VHH monoclonal Antibody
cat.: HA1129
| Product Type: | Alpaca monoclonal IgG, secondary antibodies |
|---|---|
| Species reactivity: | Rabbit |
| Applications: | IF-Cell, IHC-Fr |
| Clonality: | Monoclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Fc region of Rabbit IgG. |
| Recommended Dilutions:
IF-Cell IHC-Fr |
1:500-1:1,000 1:500-1:1,000 |
Images
|
Fig1:
Immunocytochemistry analysis of Hela cells labeling Vimentin with Rabbit anti-Vimentin antibody (HA721174) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (HA721174) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Alpaca anti-Rabbit IgG FC (iFluor™ 488, HA1129) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
|
Fig2:
Immunocytochemistry analysis of HeLa cells labeling Skp2 with Rabbit anti-Skp2 antibody (HA500427) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Skp2 antibody (HA500427) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Alpaca anti-Rabbit IgG FC (iFluor™ 488, HA1129) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.