iFluor™ 750 Conjugated Goat Anti-Rabbit IgG (H+L) Goat Polyclonal Antibody
cat.: HA1134
| Product Type: | Goat polyclonal IgG, secondary antibodies |
|---|---|
| Species reactivity: | Rabbit |
| Applications: | IF-Cell, IF-Tissue, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Rabbit IgG (H+L). |
| Recommended Dilutions:
IF-Cell IF-Tissue FC |
1:500-1:1,000 1:500-1:1,000 1:1,000 |
Images
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Fig1:
Flow cytometric analysis of HeLa cells labeling GAPDH. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1601-4, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 750 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1134) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.