iFluor™ 555 Conjugated Goat Anti-Rabbit IgG (H+L) Goat Polyclonal Antibody
cat.: HA1135
| Product Type: | Goat polyclonal IgG, secondary antibodies |
|---|---|
| Species reactivity: | Rabbit |
| Applications: | IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Rabbit IgG (H+L). |
| Recommended Dilutions:
IF-Cell FC |
1:1,000 1:1,000 |
Images
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Fig1:
Immunocytochemistry analysis of HeLa cells labeling Beta tubulin with Rabbit anti-Beta tubulin antibody (ET1602-4) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Beta tubulin antibody (ET1602-4) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 555, HA1135) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, green) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig2:
Flow cytometric analysis of HeLa cells labeling GAPDH. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1601-4, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 555 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1135) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.