iFluor™ 594 Conjugated Goat anti-Rat IgG(H+L) Goat Polyclonal Antibody
cat.: HA1136
| Product Type: | Goat polyclonal IgG, secondary antibodies |
|---|---|
| Species reactivity: | Rat |
| Applications: | IF-Tissue, IHC-Fr, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Rat IgG(H+L). |
| Recommended Dilutions:
IF-Tissue IHC-Fr IF-Cell FC |
1:500 1:500 1:500 1:500 |
Images
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Fig1:
Application: IF-Tissue Species: Mouse Site: Cerebellum Sample: Paraffin-embedded section Primary Antibody: HA601414 (1:500) Secondary Antibody Concentration: 1: 500 |
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Fig2:
Application: IF-Tissue Species: Mouse Site: Cerebellum Sample: Paraffin-embedded section Primary Antibody: HA601413 (1/1,000) Secondary Antibody Concentration: 1/500 |
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Fig3:
Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: Cerebellum Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA601414, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rat IgG (iFluor™ 594, HA1136, 1/500), 1.5 hours at room temperature. |
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Fig4:
Application: Immunocytochemistry (IF-cell) Species: Mouse Sample: C2C12 (Mouse myoblast) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA601406, 1/1,000, overnight at 4℃. Secondary antibody: Goat Anti-Rat IgG (iFluor™ 594, HA1136, 1/500), 45 minutes at room temperature. Counterstain: beta Tubulin (ET1602-4, Green), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.