iFluor™ 647 Conjugated Goat anti-Chicken IgY Goat Polyclonal Antibody
cat.: HA1142
| Product Type: | Goat polyclonal IgG, secondary antibodies |
|---|---|
| Species reactivity: | Chicken |
| Applications: | IHC-Fr, IF-Tissue, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Chicken IgY protein. |
| Recommended Dilutions:
IHC-Fr IF-Tissue IF-Cell |
1:1,000 1:1,000 1:250-1:500 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: cerebellum Sample: Frozen section Primary antibody (HA601471) concentration: 1/200 Secondary Antibody (HA1142) concentration: 1/1,000 Antigen retrieval: Not required |
|
Fig2:
Application: IF-Tissue Species: Mouse Site: cerebellum Sample: Paraffin-embedded section Primary antibody (HA601471) concentration: 1/200 Secondary Antibody (HA1142) concentration: 1/1,000 |
|
Fig3:
Immunocytochemistry analysis of C6 cells labeling Iba1 with Chicken anti-Iba1 antibody (HA601376) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Chicken anti-Iba1 antibody (HA601376) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Chicken IgY (iFluor™ 647, HA1142) was used as the secondary antibody at 1/250 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.