iFluor™ 647 Conjugated Goat anti-Guinea pig IgG Goat Polyclonal Antibody
cat.: HA1147
| Product Type: | Goat polyclonal IgG, secondary antibodies |
|---|---|
| Species reactivity: | Guinea pig |
| Applications: | IHC-Fr, IF-Tissue, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Guinea pig IgG protein. |
| Recommended Dilutions:
IHC-Fr IF-Tissue IF-Cell FC |
1:500 1:500 1:1,000 1:200-1:500 |
Images
|
Fig1:
Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: Brain Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA601368, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 647, HA1147, 1/500), 1.5 hours at room temperature. |
|
Fig2:
Application: Immunofluorescence (IF-tissue) Species: Mouse Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA601368, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 647, HA1147, 1/500), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.