iFluor™ 680se Conjugated Goat anti-rabbit IgG polyclonal Antibody(For Odyssey system)
cat.: HA1150
| Product Type: | Goat polyclonal IgG, secondary antibodies |
|---|---|
| Species reactivity: | Rabbit |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Rabbit IgG (H+L). |
| Recommended Dilutions:
WB |
1:5,000-1:10,000 |
Images
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Fig1:
Western blot analysis of GAPDH and HSP90 on different lysates at 1/20,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 10μM MG-132 for 6 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 10μM MG-132 for 8 hours cell lysate Lane 5: C6 cell lysate Lane 6: C6 treated with 25μM MG-132 for 4 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 37/90 kDa Observed band size: 37/90 kDa 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (GAPDH / HSP90) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - iFluor™ 680se Secondary Antibody (HA1150) at 1/5,000 dilution was used for 1 hour at room temperature and then imaged using the Licor Odyssey CLx. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.