iFluor™ 555 Conjugated Goat anti-mouse IgG polyclonal Antibody
cat.: HA1151
| Product Type: | Goat polyclonal IgG, secondary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | IF-Tissue, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Mouse IgG (H+L). |
| Recommended Dilutions:
IF-Tissue IF-Cell FC |
1:1,000 1:1,000 1:1,000 |
Images
|
Fig1:
Application: IF-Tissue Species: Human Site: kidney Sample: Paraffin-embedded section Primary antibody (Vimentin, EM0401) concentration: 1/400 Secondary Antibody (HA1151) concentration: 1/1,000 |
|
Fig2:
Application: IF-Tissue Species: Mouse Site: brain Sample: Paraffin-embedded section Primary antibody (Beta III Tubulin, M0805-8) concentration: 1/200 Secondary Antibody (HA1151) concentration: 1/1,000 |
|
Fig3:
Application: IF-Tissue Species: Rat Site: skin Sample: Paraffin-embedded section Primary antibody (Cytokeratin 14, EM1901-33) concentration: 1/200 Secondary Antibody (HA1151) concentration: 1/1,000 |
|
Fig4:
Flow cytometric analysis of HeLa cells labeling HSP60. Cells were fixed and permeabilized. Then stained with the primary antibody (EM00704, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 555 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1151) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
|
Fig5:
Immunocytochemistry analysis of HeLa cells labeling beta Tubulin with Mouse anti-beta Tubulin antibody (M1305-2) at 1/500 dilution, Goat Anti-Mouse IgG H&L (iFluor™ 555, HA1151) was used as the secondary antibody at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-beta Tubulin antibody (M1305-2) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 555, HA1151) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.