Human GFRAL/GFR alpha-like Protein
cat.: HA210111
| Product name: | Human GFRAL/GFR alpha-like Protein |
|---|---|
| Catalog No.: | HA210111 |
| Purity: | > 95% as determined by Tris-Bis PAGE > 95% as determined by HPLC |
| Endotoxin: | Less than 1EU per μg by the LAL method. |
| Formulation: | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).Normally 8% trehalose is added as protectant before lyophilization. |
| Background: | GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. |
| Source: | HEK293 |
| Tag: | C-His-Avi |
| Storage: | -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| Reconstitution: | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Images
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Fig1: Recombinant GFRAL Protein on Tris-Bis PAGE under reduced conditions.The purity is greater than 95% |
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Fig2: The purity of Human GFRAL is greater than 95% as determined by SEC-HPLC. |
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Fig3: Immobilized Human GFRAL, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human GDF15, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.