HA210543

Product name:	Human Cripto-1/TDGF1, C-His, Flag Tag
Catalog No.:	HA210543
Bio-Activity:	Testing in progress.
Purity:	>95% as determined by SDS-PAGE.
Endotoxin:	Less than 1.0 EU per μg by the LAL method.
Formulation:	Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Background:

Teratocarcinoma-derived growth factor (TDGF)-1 gene encodes a protein known as cripto-1 (Cripto). GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated TDGF1 acts as a Nodal coreceptor in cis. Shedding of TDGF1 by TMEM8A modulates Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells. Cripto is first expressed in the forming mesoderm during gastrulation but later in development the expression is restricted to the truncus arteriosus of the developing heart. This suggests that Cripto mediates the progression of epiblastic cells that give rise to the mesoderm. In the adult animal it is expressed at low levels in the spleen, heart, lung and brain. Cripto overexpression is characteristic of human gastric and colorectal carcinomas.

Source:

HEK293

Storage:

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution:

Reconstitute at 250 μg/ml in sterile water.