HA210700

Product name:	Human DOT1L, N-Strep II, C-His Tag
Catalog No.:	HA210700
Bio-Activity:	Testing in progress.
Purity:	>95% as determined by SDS-PAGE.
Endotoxin:	Less than 1.0 EU per μg by the LAL method.
Formulation:	Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Background:

DOT1L (mammalian counterpart of yeast Dot1) is a 1537 amino acid (aa), 165 kDa (predicted) nuclear non-SET domain histone H3K79 methyltransferase. It is involved in developmental gene transcription and maintenance of chromatin structure, and may promote mixed lineage leukemia. Within the sequence used as an immunogen, human DOT1L shares 97% and 94% aa identity with mouse and rat DOT1L, respectively. Two human isoforms affect this region: a 1467 aa form has an alternate start site at aa 71, while a 1513 aa form lacks aa 89-112. A third isoform of 1739 aa has a 202 aa C-terminal extension.

Source:

E.coli

Storage:

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution:

Reconstitute at 250 μg/ml in sterile water.