Mouse MCSF/CSF-1, C-His Tag (ECD) Protein (Active)
cat.: HA210725
| Product name: | Mouse MCSF/CSF-1, C-His Tag (ECD) |
|---|---|
| Catalog No.: | HA210725 |
| Bio-Activity: | Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells.The ED50 for this effect is 0.5-3 ng/mL |
| Purity: | >95% as determined by SDS-PAGE. |
| Endotoxin: | Less than 1.0 EU per μg by the LAL method. |
| Formulation: | Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol. |
| Background: | Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. |
| Source: | HEK293 |
| Storage: | Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Reconstitution: | Reconstitute at 250 μg/ml in sterile water. |
Images
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Fig1: Protein on SDS-PAGE under reducing (R) condition. |
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Fig2: 40ng/mL Recombinant Mouse M-CSF (Catalog # HA210725) and 50ng/mL Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # HA210603) induces osteoclast differentiation of the mouse bone marrow cell at day4. |
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Fig3: Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells.The ED50 for this effect is 0.5-3 ng/mL. |
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Fig4: Mouse BMMC were cultured in RPMI1640 containing Mouse M-CSF for 6 days. |
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Fig5: Mouse BMMC were cultured in RPMI1640 containing Mouse M-CSF for 6 days. |
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Fig6: Mouse BMMC were cultured in RPMI1640 containing Mouse M-CSF for 6 days. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.