HA210731

Product name:	Human IL-17A, Tag Free
Catalog No.:	HA210731
Bio-Activity:	Measured by its ability to induce IL-6 secretion by NIH/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 3-10 ng/mL.
Purity:	>90% as determined by SDS-PAGE.
Endotoxin:	Less than 1.0 EU per μg by the LAL method.
Formulation:	Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Background:

Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold. Mature human IL‑17A shares 60% amino acid sequence identity with mouse and rat IL‑17A. IL‑17A is secreted by Th17 cells, gamma /δ T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells. It forms disulfide‑linked homodimers as well as disulfide‑linked heterodimers with IL‑17F. IL‑17A exerts its effects through the transmembrane IL‑17 RA in complex with IL‑17 RC or IL‑17 RD. Both IL‑17 RA and IL‑17 RC are required for responsiveness to heterodimeric IL‑17A/F. IL‑17A promotes protective mucosal and epidermal inflammation in response to microbial infection. It induces chemokine production, neutrophil influx, and the production of antibacterial peptides.

Source:

HEK293

Storage:

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution:

Reconstitute at 250 μg/ml in sterile water.

	Fig1: Protein on SDS-PAGE under reducing (R) condition.
	Fig2: Measured by its ability to induce IL-6 secretion by NIH/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 3-10 ng/mL.