HA210735

Product name:	Mouse LIF, C-His Tag
Catalog No.:	HA210735
Bio-Activity:	Mouse LIF can replace fibroblast feeding layer or other conditioned medium under the condition of 1000 U/mL, maintain the undifferentiated state of mouse embryonic stem cells ES.W4 and promote cell proliferation.
Purity:	>95% as determined by SDS-PAGE.
Endotoxin:	Less than 1.0 EU per μg by the LAL method.
Formulation:	Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Background:

The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

Source:

HEK293

Storage:

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution:

Reconstitute at 250 μg/ml in sterile water.

	Fig1: Protein on SDS-PAGE under reducing (R) condition.
	Fig2: Mouse LIF can replace fibroblast feeding layer or other conditioned medium under the condition of 1000 U/mL, maintain the undifferentiated state of mouse embryonic stem cells ES.W4 and promote cell proliferation.