HA210876

Product name:	Mouse TRANCE/RANK L/TNFSF11, N-GST, His Tag
Catalog No.:	HA210876
Bio-Activity:	40ng/mL Recombinant Mouse M-CSF (Catalog # HA210725) and 50ng/mL Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # HA210876) induces osteoclast differentiation of the mouse bone marrow cell at day5.
Purity:	>90% as determined by SDS-PAGE.
Endotoxin:	Less than 1.0 EU per μg by the LAL method.
Formulation:	Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Background:

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca 2+ resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation.

Source:

E.coli

Storage:

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution:

Reconstitute at 100 μg/ml in sterile water.

	Fig1: Protein on SDS-PAGE under reducing (R) condition.
	Fig2: 40ng/mL Recombinant Mouse M-CSF (Catalog # HA210725) and 50ng/mL Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # HA210876) induces osteoclast differentiation of the mouse bone marrow cell at day5.
	Fig3: 50ng/mL Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # HA210876) induces osteoclast differentiation of the RAW 264.7 mouse monocyte/macrophage cell line at day5.