Human FGF-4, C-His Tag Protein (Active)
cat.: HA210946
Product name: Human FGF-4, C-His Tag
Catalog No.: HA210946
Bio-Activity: Measured in a cell proliferation assay using NIH/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 6-30 ng/mL.
Purity: >95% as determined by SDS-PAGE.
Endotoxin: Less than 1.0 EU per μg by the LAL method.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
Background: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified by its oncogenic transforming activity. This gene and FGF3, another oncogenic growth factor, are located closely on chromosome 11. Co-amplification of both genes was found in various kinds of human tumors. Studies on the mouse homolog suggested a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway.
Source: E.coli
Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
Reconstitution: Reconstitute at 250 μg/ml in sterile water.
Images
HA210946_1.jpg Fig1: Protein on SDS-PAGE under reducing (R) condition.
HA210946_2.jpg Fig2: Measured in a cell proliferation assay using NIH/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 6-30 ng/mL.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.