HA211017

Product name:	Human Neuropilin-1/NRP1, C-His Tag (ECD)
Catalog No.:	HA211017
Bio-Activity:	Immobilized Recombinant Human VEGF165, Tag Free(Cat:HA210784) at 2 μg/mL (50 μL/well) can bind Recombinant Human Neuropilin-1/NRP1, C-His Tag (ECD)(Cat:HA211017), the EC50 is 30-120 ng/mL.
Purity:	>95% as determined by SDS-PAGE.
Endotoxin:	Less than 1.0 EU per μg by the LAL method.
Formulation:	Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Background:

Neuropilin is a type I transmembrane receptor that has been implicated in aspects of axon growth and guidance and has been shown to act as a high affinity receptor for class III semaphorins and vascular endothelial growth factor (VEGF). A closely related protein, neuropilin-2, shares a common domain structure and signifcant homology with neuropilin and also acts as a receptor for the class III semaphorins and VEGF. Both neuropilins are involved in regulating many physiological pathways including axonal guidance and angiogenesis, however they exhibit differential expression in the adult vasculature. Neuropilin-2 is polysialylated and expressed on the surface of dendritic cells. It is also expressed by venous and lymphatic endothelium. Neuropilin is expressed predominantly by arterial endothelium.

Source:

HEK293

Storage:

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution:

Reconstitute at 250 μg/ml in sterile water.

	Fig1: Protein on SDS-PAGE under reducing (R) condition.
	Fig2: Immobilized Recombinant Human VEGF165, Tag Free(Cat:HA210784) at 2 μg/mL (50 μL/well) can bind Recombinant Human Neuropilin-1/NRP1, C-His Tag (ECD)(Cat:HA211017), the EC50 is 30-120 ng/mL.